Analytical Chemistry Lab — Titrations, Spectrophotometry & Chromatography
Chemistry
PhD-level analytical chemistry: potentiometric acid-base titration curves with buffer region analysis (Henderson-Hasselbalch, polyprotic systems), Beer-Lambert law and UV-Vis spectrophotometry (calibration, limit of detection, matrix effects), high-performance liquid chromatography (HPLC) resolution equation, the van Deemter equation for plate-height optimisation, and method validation (linearity, precision, accuracy, specificity).
Learning Objectives
- Derive and compute acid-base titration curves for strong acid-strong base, weak acid-strong base, and polyprotic systems
- Apply the Henderson-Hasselbalch equation to predict buffer pH and identify the optimal buffer pH range
- Use the Beer-Lambert law to construct calibration curves and compute analyte concentration with error propagation
- Apply the van Deemter equation to optimise HPLC flow rate for maximum column efficiency (minimum HETP)
- Validate an analytical method using ICH guidelines: linearity, precision (repeatability, reproducibility), accuracy, LOD, LOQ
Lessons
Quick Practice
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